Home > Catalogo generale > Culture media and ISO confirmation tests > TSC Agar: base medium for the enumeration of sulfite reducing clostridia in foodstuff (ISO15213) and water (ISO26461)

TSC Agar: base medium for the enumeration of sulfite reducing clostridia in foodstuff (ISO15213) and water (ISO26461)

  • Brand: Sifin
  • Format: 500 g
  • Code: TN1241
  • Technology: Culture media and ISO confirmation tests
  • Application: Food/Water microbiology
  • Tag/Filter: Sulfite reducing clostridia

TSC is a medium used for the detection and enumeration of sulfite-reducing anaerobic bacteria in food products and in water according to many food and water microbiology national and international standards. The medium is available as dehydrated powder or as ready-to-melt bottles to be supplemented with D-cycloserin and when necessary with egg yolk. The principle of the medium relies on the ability of Clostridium to reduce sulfites to sulphide which, in the presence of ferric citrate, forms a black precipitate of iron sulfide around the colonies. Due to the presence of D-cycloserin, the medium inhibits other bacteria. The colonies of sulfite-reducing anaerobes are black with a black halo. Only dishes presenting colonies that are clearly distinct from one another should be used for enumeration. Incubation at 46°C enhances the selectivity of the medium with regard to Clostridium perfringens. For the specific enumeration of C. perfringens, further tests should be carried out on a representative number of typical colonies. When egg yolk is present in the medium, colonies of C. perfringens are black and usually surrounded by an opaque halo caused by the lecithinase of this bacterium.


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TSC Agar: base medium for the enumeration of sulfite reducing clostridia in foodstuff (ISO15213) and water (ISO26461)
  • Brand: Sifin
  • Format: 500 g
  • Code: TN1241
  • Technology: Culture media and ISO confirmation tests
  • Application: Food/Water microbiology
  • Tag/Filter: Sulfite reducing clostridia


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