TBX Agar – Selective chromogenic medium for direct enumeration of Escherichia coli in food and feed acc. to ISO16649
- Format: 500 g
- Code: TN1259
- Technology: Culture media and ISO confirmation tests
- Application: Food/Water microbiology
- Tag/Filter: Escherichia coli and coliforms
Selective chromogenic medium for direct enumeration (without colonies confirmation) of Escherichia coli in products intended for human and animal consumption according to ISO16649. The principle of the medium relies on detection of the ß-D-glucuronidase of Escherichia coli. The medium contains a complex (chromogen linked to a sugar) called 5-bromo-4-chloro-3-indolyl-ß-glucuronide (BCIG) which is specific to ß-D-glucuronidase. Absorbed by the target micro-organism, the complex is hydrolysed by this enzyme. The sugar is consumed by the bacteria and the chromogen agent accumulates in this same cell, giving E. coli colonies a blue or blue-green color. Due to the conjugated action of high temperature and bile salts, this medium is selective with regard to Gram positive and interfering flora. After the incubation period, count any characteristic E. coli colonies, which are blue or blue-green. Other colonies are white/beige. SIFIN TBX is available as dehydrated powder.
- Format: 500 g
- Code: TN1259
- Technology: Culture media and ISO confirmation tests
- Application: Food/Water microbiology
- Tag/Filter: Escherichia coli and coliforms
