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Colony PCR

Identification of isolated colonies and ISO 7218

All horizontal methods for the search for pathogenic bacteria include, within the ISO standard, a paragraph related to the methods of identification / confirmation of presumed positive colonies. ISO 7218, one of the fundamental standards for the regulation of microbiological analyzes, indicates the operating procedures for the identification of colonies and includes molecular methods.

All PATHfinder Real-Time PCR kits proposed by Generon are based on international scientific literature, therefore they can be used for the identification of colonies within an ISO17025 accreditation scheme based on international standards.

Target
Alicyclobacillus acidocaldariusEscherichia coli O:26 [wzx]
Alicyclobacillus acidoterrestrisEscherichia coli O:45 [wzx]
Alicyclobacillus spp.Escherichia coli fattori di virulenza (stx1, stx2, eae)
Bacillus cereus e fattori di virulenzaLegionella pneumophila
Campylobacter termotollerantiLegionella pneumophila Sierogruppo 1
Clostridium botulinumLegionella spp.
Clostridium botulinum A, B, E, FListeria monocytogenes
Clostridium PerfringensListeria spp.
Cronobacter spp.Pseudomonas Aeruginosa
EnterobacteriaceaeSalmonella spp.
Escherichia coliShigella spp.
Escherichia coli H:4 [fliC]Staphylococcus aureus
Escherichia coli H:7 [fliC]Vibrio cholerae
Escherichia coli O:103 [wzx]Vibrio parahemolyticus e fattori di virulenza (tdh/trh)
Escherichia coli O:104 [wzx]Vibrio vulnificus
Escherichia coli O:111 [wbdL]Xanthomonas Campestris
Escherichia coli O:121 [wzx]Yersinia Enterocolitica
Escherichia coli O:145 [ihp1]Yersinia pseudotubercolosis
Escherichia coli O:157 [rfbE] 

Advantages: Compared to biochemical and serological methods, when usable, PCR has innumerable advantages both in terms of practicality and in terms of cost / time savings for the operator:

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