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Neogen One-Broth-One-Plate Listeria isolation medium – ISO 16140 validated

  • Format: 20 x 90 mm Petri
  • Code: NCM3000-20
  • Technology: Chromogenic agar
  • Application: Food/Water microbiology
  • Tag/Filter: Listeria,Listeria

Harlequin Listeria Chromogenic Agar (according to the formulation of Ottaviani and Agosti) is a selective medium for the isolation and presumptive identification of Listeria monocytogenes from foodstuffs and related materials as described in ISO 11290-1:2017. Lithium chloride in the base medium and supplementary antimicrobial compounds ceftazidime, polymyxin, nalidixic acid, and cycloheximide provide the medium's selectivity. Chromogenic activity is a result of a chromogenic substrate for the detection of the ?-glucosidase enzyme, common to all Listeria spp. and a few strains of Enterococci and Bacilli. The specific differential activity of this agar is obtained with a proprietary lecithin substrate for the detection of the phospholipase enzyme present in the L. monocytogenes colonies growing on this media. This enzyme activity will result in a halo of precipitation surrounding the target colonies. Combining both the chromogenic and phospholipase enzyme reactions makes it possible to differentiate Listeria monocytogenes (blue colonies surrounded by an opaque halo) from other Listeria spp. (blue colonies without an opaque halo). Harlequin Listeria Chromogenic Agar forms with the enrichment broth LESS Plus, Neogen's innovative One-Broth-One-Plate Listeria (OBOP-L) workflow that has been validated according to ISO 16140-2 and certified by AFNOR allowing the detection of Listeria in food and environmental samples, using just one enrichment broth and one agar plate in 48 hours.


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Neogen One-Broth-One-Plate Listeria isolation medium – ISO 16140 validated
  • Format: 20 x 90 mm Petri
  • Code: NCM3000-20
  • Technology: Chromogenic agar
  • Application: Food/Water microbiology
  • Tag/Filter: Listeria,Listeria


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