RiboFlow – Rapid test for the molecular detection and differentiation of Alicyclobacillus spp. and Alicyclobacillus acidoterrestris

The genus Alicyclobacillus consists of Gram-positive, spore-forming thermoacidophilic bacteria, which are typically found in soil. Alicyclobacillus spp. spores can contaminate fruit during production. The spores survive the pasteurization process which, indeed, determines their germination. Although not pathogenic, vegetative cells cause unpleasant flavors (via guaiacol or halogenated phenols) in fruit juices, concentrates and tomato products. The species Alicyclobacillus acidoterrestris is the most observed cause of deterioration, fermentation processes occur without gas production and therefore do not show up in the swelling of the containers.
SY-LAB Riboflow Ali-Twin provides fast and accurate detection for Alicyclobacillus spp. and Alicyclobacillus acidoterrestris. This test combines the specificity of PCR with the ease of use of lateral flow and allows to detect in less than 30 'the presence of Alicyclobacilli in an enrichment broth or in a polycarbonate filter on which the bacteria present in a water sample have been collected. Similarly, the kit genetically identifies as Alicyclobacilli bacterial colonies isolated on any solid agar medium.

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