Salmonella Omni-O antiserum: agglutination test for the confirmation of suspect Salmonella isolated colonies

Agglutinating Salmonella Omni-O antiserum (A-60) allows presumptive identification of O-agglutinable strains of Salmonellae belonging to groups A to 60 from colonies isolated on agar medium. The test is validated to confirm presumptive Salmonella colonies isolated on RAPID’Salmonella agar. The test is based on agglutination by omnivalent Salmonella antiserum of bacteria possessing the corresponding antigens. Salmonella Omni-O antiserum is composed of a mixture of crude sera obtained by immunization of rabbits with reference strains. It contains agglutinins directed against O antigenic factors of Salmonellae belonging to groups A to 60. No agglutination should be observed with the normal saline solution. If agglutination is observed, it corresponds to a self-agglutinating strain and reaction with the antiserum therefore cannot be interpreted. A positive reaction corresponds to the appearance of agglutination with the antiserum in less than 30 seconds. When the reaction is delayed or when the suspension retains a homogeneous, milky appearance, the result is negative. Agglutination of a strain of Salmonella with Omni-O antiserum indicates that the strain is O-agglutinable and can be serotyped with the aid of specific sera (polyvalent then monovalent). Cross-reactions are possible with the Escherichia, Shigella, Citrobacter and Proteus due to the partial antigenic identity of these bacteria.

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