Home > Catalogo generale > Culture media and ISO confirmation tests > ONPG: beta-galactosidase test for confirmation of suspicious Salmonella colonies acc. to ISO6579

ONPG: beta-galactosidase test for confirmation of suspicious Salmonella colonies acc. to ISO6579

  • Brand: Bio-Rad
  • Format: 50 Test
  • Code: BR3553822
  • Technology: Culture media and ISO confirmation tests
  • Application: Food/Water microbiology
  • Tag/Filter: Salmonella

Certain Enterobacteria possess: the enzyme necessary for the use of lactose by the bacteria (permease); the enzyme splitting the lactose molecule into glucose and galactose (galactosidase). If the first of these two activities is deficient or absent, a potentially lactose-positive bacteria - because it possesses a galactosidase – would be unable to express this character and would appear lactose-negative. Acidification of a lactose medium will manifest only later, if a reverse mutant appears, or perhaps not occur at all. ONPG in solution is colourless. The molecule is split, like lactose, by galactosidase, releasing the orthonitrophenol, yellow in solution. This reaction is carried out in buffered solution. The disks are ready to use and impregnated with ONPG + buffer.


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ONPG: beta-galactosidase test for confirmation of suspicious Salmonella colonies acc. to ISO6579
  • Brand: Bio-Rad
  • Format: 50 Test
  • Code: BR3553822
  • Technology: Culture media and ISO confirmation tests
  • Application: Food/Water microbiology
  • Tag/Filter: Salmonella


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