Bio-Rad RAPID’Sakazakii – chromogenic medium AFNOR validated for 48 hrs detection

Bio-Rad RAPID'Sakazakii Medium is a selective chromogenic agar that is intended for the isolation and detection of Cronobacter spp. (formerly classified as Enterobacter sakazakii). The most common source of Cronobacter spp. contamination is powdered infant formula. RAPID'Sakazakii Agar is offered as ready-to-use 90 mm plates or as a dehydrated agar base. RAPID'Sakazakii medium is AFNOR Certified according to ISO 16140 versus ISO22964 standard method for the detection of Cronobacter spp. in infant milk powders, hence, it allows a straightforward accreditation according to ISO17025. The high specificity and high selectivity of RAPID'Sakazakii medium is based on a specific enzymatic activity common to all species of Cronobacter leading to an easy-to-read chromogenic reaction with the formation of typical turquoise-colored colonies. The medium includes compounds carefully selected to promote the growth of Cronobacter spp. while suppressing interfering flora, thus the primary enrichment can be performed in buffered peptone water with no need for a selective enrichment in mLST + Vancomycin. Competing bacteria, if not completely inhibited, will form light purple or gray colonies. Negative results can be obtained in less than 42 hr, positive colonies can be confirmed using standard biochemical method or iQ-Check Cronobacter PCR Detection Kit (BR3578137) and reciprocally iQ-Check PCR Kit is validated to confirm suspect positive colonies grown on the Rapid' medium.