Bio-Rad RAPID’Listeria spp. – chromogenic medium AFNOR validated for 48 hrs detection

When Listeria gets into food, it often comes from environmental contamination. Finding sources of contamination by Listeria spp. in the processing plant is the first step towards preventing Listeria monocytogenes contamination of food. A proactive, aggressive environmental monitoring program is needed as a first step in preventing contamination. RAPID'Listeria spp. Medium is an affordable selective chromogenic agar for the detection and enumeration of Listeria spp. in food and environmental samples. It is offered as a dehydrated agar base to be supplemented or as ready-to-use plates. RAPID'Listeria spp. is AFNOR Certified according to norm ISO16140 versus the ISO11290 standard method, hence, it allows a straightforward accreditation according to ISO17025 with no restriction-of-use. The protocol is based on the ONE Broth ONE Plate approach and provides FAST RESULTS detecting Listeria in less than 24 hrs after the enrichment or the plating. The confirmation can be conveniently done with many validated tests. L. monocytogenes enumeration can be performed in 24 hrs without confirmation. RAPID'Listeria spp. high selectivity prevents growth of interfering flora. Reading and differentiation are easy due to the contrast between blue-green Listeria colonies and the whitish agar. Single spot inoculation on RAPID'L.mono agar (+Gram and catalase tests) or on Palcam has been validated for confirmation. The medium is also validated to confirm positive tests obtained with the iQ-Check Listeria PCR detection kit and reciprocally PCR can be used to confirm presumptive Listeria colonies grown on medium.

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