Bio-Rad RAPID’E.Coli O157 – chromogenic medium AFNOR validated for 48 hrs detection

Bio-Rad RAPID'E.coli O157:H7 Medium is a selective chromogenic agar for the detection, isolation, and presumptive identification of Escherichia coli O157:H7 in human food products and environmental samples. RAPID'E.coli O157:H7 Medium is available as a dehydrated agar and provides laboratories with a rapid and cost-effective solution for E. coli O157:H7 detection, given that a single plate is required for each sample. Results can be obtained in 48 hr, after a pre-enrichment and an immunoseparation step. The latex test for confirmation has been validated for use on isolated colonies grown on RAPID'E.coli plates. RAPID'E.coli O157:H7 medium is AFNOR Certified according to ISO 16140 versus ISO16654 standard method for the detection of E.coli O157:H7, hence, it allows a straightforward accreditation according to ISO17025. RAPID'E.coli O157:H7 medium permits the detection of E. coli O157 serotype H7 using specific chromogenic substrates and biochemical activities. Typical E.coli O157:H7 colonies are characteristically convex 1–2 mm in diameter and dark blue to black in color - Atypical GLUC + E. coli O157:H7 are detected and present as dark blue to black colonies - Atypical SORB + E. coli O157:H7 are detected and present as blue to turquoise blue colonies. RAPID'E.coli O157:H7 Medium is very specific. Most other bacteria and yeasts are inhibited or produce a different color. The selectivity of RAPID'E.coli O157:H7 Medium can be improved by adding selective agents such as novobiocin and potassium tellurite. RAPID'E.coli O157:H7 can be used also to confirm positive results obtained with the iQ-Check E. coli O157:H7 PCR Detection Kit (BR3578114) and reciprocally the iQ-Check PCR kit is also validated to confirm suspect positive colonies grown on the Rapid' medium.

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