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Bio-Rad iQ-Check – Real-Time PCR kit for Aspergillus detection

  • Brand: Bio-Rad
  • Format: 96 Test
  • Code: BR12010806
  • Technology: Real-Time PCR
  • Application: Food/Water microbiology
  • Tag/Filter: Spoilage microrganisms

Due to increasing state-level legislation concerning medicinal and recreational marijuana across the United States and Canada, consumers now have access to cannabis edibles and inhalables for consumption that can be contaminated by yeast and mold like Aspergillus spp. These molds are a leading cause of aspergillosis infections in humans. A trend for a microbial pathogen detection system in cannabis and its derivatives is emerging. Due to concerning health impacts, challenges are focused on effective and efficient screening detection. Gold standard methods for yeast and mold testing, such as the FDA BAM - Chapter 18 (1), typically lack quantitative capability, rely on microbiological/ biochemical identification and are labor intensive. These current methods require at least 5 days for results and subjective interpretation for the screening of true positives and are not specific for Aspergillus species. The iQ-Check Aspergillus kit uses an optimized system of primers and probes to ensure high specificity. It is designed as a multiplex reaction targeting the four main species (A. fumigatus, A. flavus and A. niger, A. terreus) linked to aspergillosis that also enables differentiation of A. terreus in cannabis products and includes an internal inhibition control that is amplified in parallel with the target DNA for assuring a reliable negative result. Due to the sensitivity and specificity of PCR, results can be obtained in as little as 48 hrs after a single enrichment in buffered peptone water. All the iQ-Check Kits include the DNA extraction buffer and share the same amplification protocol, hence they can be run on in parallel on the same plate using low- or high-throughput instruments, depending on the laboratory's needs. The iQ-Check workflow can be automatized using the iQ-Check Prep System, a robotic liquid handling platform performing DNA extraction and PCR plate setup.


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Bio-Rad iQ-Check – Real-Time PCR kit for Aspergillus detection
  • Brand: Bio-Rad
  • Format: 96 Test
  • Code: BR12010806
  • Technology: Real-Time PCR
  • Application: Food/Water microbiology
  • Tag/Filter: Spoilage microrganisms


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