Bio-Rad iQ-Check – PCR Real-Time kit for rapid detection of Salmonella typhimurium – Validated AOAC

Though the incidence of human salmonellosis is decreasing in some areas, millions of cases are still reported worldwide each year. Salmonella enterica subspecies enterica serovar Typhimurium is one of the most frequently implicated serovars. Pork and poultry feed have been identified as among the most common food sources linked to these S. Typhimurium infections. Regulations have been implemented in the EU to monitor Salmonella spp. and specific serotypes, including S. Typhimurium, in poultry from the primary production stage to food products. The iQ-Check Salmonella Typhimurium PCR Detection Kit is based on the fast, sensitive, and proven technology of real-time PCR. The kit is intended for the rapid and specific detection of S. Typhimurium in food and environmental samples (including primary production environments) within a few hours after microbiological enrichment. Results can be obtained in 3 hrs following after 16 hours single enrichment in a nonselective buffered peptone water. Positive samples can be confirmed starting from the same broth using Bio-Rad RAPID'Salmonella Agar. iQ-Check Salmonella Typhimurium Kit uses an optimized multiplex system of primers and probes to ensure high specificity and include an internal inhibition control (HEX) that is amplified in parallel with the target DNA (FAM) for a reliable negative result. The iQ-Check Free DNA Removal Solution completes and optimizes the method by removing free DNA from food and environmental samples. The iQ-Check Salmonella Typhimurium kit has been validated and certified according to AOAC as equivalent to or better than the reference method ISO6579. All the iQ-Check Kits share the same amplification protocol, hence they can be run on in parallel on the same plate using low- or high-throughput instruments, depending on the laboratory's needs. The iQ-Check workflow can be automatized using the iQ-Check Prep System, a robotic liquid handling platform performing DNA extraction and PCR plate setup.

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