Bio-Rad iQ-Check – PCR Real-Time kit for rapid detection of Listeria spp – Validated AFNOR ISO16140 – Validated AOAC

The iQ-Check Listeria spp. PCR Detection Kit is based on the fast, sensitive, and proven technology of real-time PCR. The kit is intended for detecting Listeria spp. in environmental samples and in food samples such as dairy products and fish. Due to the sensitivity and specificity of PCR, results can be obtained in as little as 24 hrs after a single enrichment in a selective medium. Positive samples can be confirmed starting from the same broth using Bio-Rad RAPID'Listeria spp. Agar. iQ-Check Listeria spp. Kit uses an optimized system of primers and probes to ensure high specificity. It is designed as a multiplex reaction that includes an internal inhibition control (HEX) that is amplified in parallel with the target DNA (FAM) for assuring a reliable negative result. The iQ-Check Free DNA Removal Solution completes and optimizes the method by removing free DNA from food and environmental samples. The iQ-Check Listeria spp. kit has been validated and certified according to ISO16140 as equivalent to or better than the reference methods ISO11290, hence it can be easily implemented in ISO17025 accredited laboratories. The kit is also approved FDA/USDA. All the iQ-Check Kits include the DNA extraction buffer and share the same amplification protocol, hence they can be run on in parallel on the same plate using low- or high-throughput instruments, depending on the laboratory's needs. The iQ-Check workflow can be automatized using the iQ-Check Prep System, a robotic liquid handling platform performing DNA extraction and PCR plate setup.