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Bio-Rad iQ-Check – PCR Real-Time kit for rapid detection of Enterobacteriacea

  • Brand: Bio-Rad
  • Format: 96 Tests
  • Code: BR12003068
  • Technology: Real-Time PCR
  • Application: Food/Water microbiology
  • Tag/Filter: Microbiological indicators

Enterobacteriaceae, often used as indicators for hygiene process criteria, is a large family of gram-negative bacteria that encompasses some of the most well-known food pathogens like Salmonella, Cronobacter, or pathogenic E. coli. For thermal processed food or ingredients, this provides an indication of the efficiency of inactivation treatment, but also as a prevention of recontamination. Testing of total Enterobacteriaceae is based on the standard ISO 21528-1 method, which is labor intensive and requires at least 2 days for the screening of negative samples. The iQ-Check Enterobacteriaceae Kit is a test based on gene amplification and detection by real-time PCR. The method is intended for the detection of Enterobacteriaceae in infant formula and related raw ingredients as well as environmental samples. The method is also a complement to iQ-Check Cronobacter Solution. Due to the sensitivity and specificity of PCR, results can be obtained in as little as 3 hrs following a single enrichment in non-selective buffered peptone water. Positive samples can be confirmed starting from the same broth using Bio-Rad RAPID'Enterobacteriaceae Agar. iQ-Check Enterobacteriaceae Kit uses an optimized system of primers and probes to ensure high specificity. It is designed as a multiplex reaction that includes an internal inhibition control (HEX) that is amplified in parallel with the target DNA (FAM) for assuring a reliable negative result. The iQ-Check Free DNA Removal Solution completes and optimizes the method by removing free DNA from food and environmental samples. All the iQ-Check Kits include the DNA extraction buffer and share the same amplification protocol, hence they can be run on in parallel on the same plate using low- or high-throughput instruments, depending on the laboratory's needs. The iQ-Check workflow can be automatized using the iQ-Check Prep System, a robotic liquid handling platform performing DNA extraction and PCR plate setup.


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Bio-Rad iQ-Check – PCR Real-Time kit for rapid detection of Enterobacteriacea
  • Brand: Bio-Rad
  • Format: 96 Tests
  • Code: BR12003068
  • Technology: Real-Time PCR
  • Application: Food/Water microbiology
  • Tag/Filter: Microbiological indicators


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