Home > Catalogo generale > Bio-Rad iQ-Check – PCR Real-Time kit for rapid detection of Cronobacter spp – Validated AFNOR ISO16140

Bio-Rad iQ-Check – PCR Real-Time kit for rapid detection of Cronobacter spp – Validated AFNOR ISO16140

  • Brand: Bio-Rad
  • Format: 96 Test
  • Code: BR3578137
  • Technology: Real-Time PCR
  • Application: Food/Water microbiology
  • Tag/Filter: Chronobacter Sakazakii

Cronobacter, formerly known as Enterobacter sakazakii, is a ubiquitous pathogen that has been isolated from food, environmental and clinical samples. It is associated with rare but fatal infant infections linked to the consumption of reconstituted powdered infant formula. The iQ-Check Cronobacter spp. PCR Detection Kit is based on the fast, sensitive, and proven technology of real-time PCR. The kit is intended for the detection of Cronobacter spp. in food, mainly infant formula and baby food, and environmental samples. Due to the sensitivity and specificity of PCR, results can be obtained in as little as 16 hrs following a single enrichment in nonselective buffered peptone water. Positive samples can be confirmed starting from the same broth using Bio-Rad RAPID'Cronobacter spp. Agar. iQ-Check Cronobacter spp. Kit uses an optimized system of primers and probes to ensure high specificity. It is designed as a multiplex reaction that includes an internal inhibition control (HEX) that is amplified in parallel with the target DNA (FAM) for assuring a reliable negative result. The iQ-Check Free DNA Removal Solution completes and optimizes the method by removing free DNA from food and environmental samples. The iQ-Check Cronobacter spp. kit has been validated and certified according to ISO16140 as equivalent to or better than the reference methods ISO22964, hence it can be easily implemented in ISO17025 accredited laboratories. All the iQ-Check Kits include the DNA extraction buffer and share the same amplification protocol, hence they can be run on in parallel on the same plate using low- or high-throughput instruments, depending on the laboratory's needs. The iQ-Check workflow can be automatized using the iQ-Check Prep System, a robotic liquid handling platform performing DNA extraction and PCR plate setup.


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Bio-Rad iQ-Check – PCR Real-Time kit for rapid detection of Cronobacter spp – Validated AFNOR ISO16140
  • Brand: Bio-Rad
  • Format: 96 Test
  • Code: BR3578137
  • Technology: Real-Time PCR
  • Application: Food/Water microbiology
  • Tag/Filter: Chronobacter Sakazakii


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