Bio-Rad iQ-Check – 4-plex PCR Real-Time kit for rapid detection of Vibrio – Validated AOAC

The iQ-Check Vibrio PCR Detection Kit is a multiplex test based on gene amplification and detection by real-time PCR. This test allows the qualitative detection of V. cholerae, V. parahaemolyticus, and V. vulnificus in food samples previously enriched by culture. The main targeted matrices are seafood, including fish and shellfish (mollusks and crustaceans). Due to the sensitivity and specificity of PCR, results can be obtained in as little as 10 hrs after a single enrichment in a selective medium. Positive samples can be confirmed starting from the same broth using agar plating. The iQ-Check Vibrio Kit uses an optimized system of primers and probes to ensure high specificity. It is designed as a multiplex reaction that includes an internal inhibition control control that is amplified in parallel with the target DNA for assuring a reliable negative result. The iQ-Check Free DNA Removal Solution completes and optimizes the method by removing free DNA in the sample. The iQ-Check Vibrio spp. kit has been validated and certified according to AOAC as equivalent to or better than the reference method ISO21872. All the iQ-Check Kits include the DNA extraction buffer and share the same amplification protocol, hence they can be run on in parallel on the same plate using low- or high-throughput instruments, depending on the laboratory's needs. The iQ-Check workflow can be automatized using the iQ-Check Prep System, a robotic liquid handling platform performing DNA extraction and PCR plate setup.

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