Home > Catalogo generale > Real-Time PCR > Bio-Rad iQ-Check – Kit Real-Time PCR per la ricerca di E.coli O157:H7 – Validato AFNOR ISO16140 – Validato AOAC

Bio-Rad iQ-Check – Kit Real-Time PCR per la ricerca di E.coli O157:H7 – Validato AFNOR ISO16140 – Validato AOAC

  • Brand: Bio-Rad
  • Format: 96 Test
  • Code: BR3578114
  • Technology: Real-Time PCR
  • Application: Food/Water microbiology
  • Tag/Filter: Cytotoxic E.coli

The iQ-Check E.coli O157:H7 PCR Detection Kit is based on the fast, sensitive, and proven technology of real-time PCR. The kit is intended for detecting E. coli O157:H7 in food samples such as raw beef and fresh produce. Due to the sensitivity and specificity of PCR, results can be obtained in little as 12 hrs following a single enrichment in a nonselective buffered peptone water. Positive samples can be confirmed starting from the same broth using Bio-Rad RAPID’ E.coli O157:H7 Agar. The kit uses an optimized system of primers and probes to ensure high specificity and eliminate cross-reactions with close strains such as E. coli O55. It is designed as a multiplex reaction that includes an internal inhibition control (HEX) that is amplified in parallel with the target DNA (FAM) for assuring a reliable negative result. The iQ-Check Free DNA Removal Solution completes and optimizes the method by removing free DNA from food and environmental samples. The iQ-Check E.coli O157:H7 PCR Detection Kit has been validated and certified according to ISO16140 as equivalent to the reference methods ISO13136, hence it can be easily implemented in ISO17025 accredited laboratories. All the iQ-Check Kits include the DNA extraction buffer and share the same amplification protocol, hence they can be run on in parallel on the same plate using low- or high-throughput instruments, depending on the laboratory’s needs. The iQ-Check workflow can be automatized using the iQ-Check Prep System, a robotic liquid handling platform performing DNA extraction and PCR plate setup.


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Bio-Rad iQ-Check – Kit Real-Time PCR per la ricerca di E.coli O157:H7 – Validato AFNOR ISO16140 – Validato AOAC
  • Brand: Bio-Rad
  • Format: 96 Test
  • Code: BR3578114
  • Technology: Real-Time PCR
  • Application: Food/Water microbiology
  • Tag/Filter: Cytotoxic E.coli


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