Home > Catalogo generale > Droplet Digital PCR > Bio-Rad dd-Check STEC – Kit per la ricerca e caratterizzazione di STEC con tecnica ddPCR

Bio-Rad dd-Check STEC – Kit per la ricerca e caratterizzazione di STEC con tecnica ddPCR

  • Brand: Bio-Rad
  • Format: 1 Unit
  • Code: BR12009999
  • Technology: Droplet Digital PCR
  • Application: Food/Water microbiology
  • Tag/Filter: Cytotoxic E.coli

Bio-Rad dd-Check STEC is a kit for detecting virulence genes from Shiga toxin-producing Escherichia coli (STEC). Pathogenic STEC is defined as the presence of both stx1 and stx2, as well as eae, in one E. coli bacterium. Unless performed on colony isolates traditional screening tests for pathogenic STEC, based on Real-Time PCR, cannot distinguish between bacteria carrying both virulence markers (true positive) and mixed cultures in which these target genes are present in different bacteria (false positive). Bio-Rad dd-Check STEC kit, which harnesses Bio-Rad’s Droplet Digital PCR (ddPCR) technology, is the first commercially available test for determining the coexistence of the STEC virulence genes in a single bacterium, in enriched cultures, providing rapid and reliable confirmation of the presence of pathogenic STEC. Since dd-Check STEC Solution accurately determines the coexistence of stx and eae in the same bacterium without additional confirmatory steps, it can return results in a few hours. Decreasing screening and confirmation turnaround time means products can be released to market faster, which is especially important for food matrices with limited shelf life. The dd-Check STEC Solution uses ddPCR technology to generate thousands of uniform nanoliter-sized droplets. Once generated, the droplets can be amplified, read, and analyzed for the colocalization of virulence genes by Bio-Rad Qx IDE Manager Software. By encapsulating a single bacterium in each droplet, the dd-Check STEC Kit can distinguish between a true positive and a negative result. As regulations classifying STEC as an adulterant continue to change, the kit provides the flexibility to adapt to new requirements. If other virulence factors are required for testing, the workflow can be updated to include additional testing.


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Bio-Rad dd-Check STEC – Kit per la ricerca e caratterizzazione di STEC con tecnica ddPCR
  • Brand: Bio-Rad
  • Format: 1 Unit
  • Code: BR12009999
  • Technology: Droplet Digital PCR
  • Application: Food/Water microbiology
  • Tag/Filter: Cytotoxic E.coli


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